You are using a browser version with limited support for CSS.To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer).Although the tree had poor resolution, and low bootstrap support across most of the topology, it provided evidence of moderate-to-strong relationships between Smed-dmd-1 and homologues in S. mediterranea do not have obvious close homologues in other species based on phylogenetic reconstructions using DM domain or BLAST comparisons using the full protein sequence.mansoni (Sm_dmd-1 and Sm_dmd-2, Gen Bank/EMBL/DDBJ accession numbers XM002575184.1 and XM002574366.1) and the liver fluke, Clonorchis sinensis (dmrt-1a and dmrt-1b, Gen Bank/EMBL/DDBJ accession numbers GAA52246.1 and GAA34150.2) (Supplementary Fig. We used whole-mount in situ hybridization (WISH) to investigate the expression of the four DM domain genes in the planarian, S. Of the four genes, only Smed-dmd-1 m RNA transcripts were detected in the reproductive system.Here, we show that proper development of the hermaphroditic reproductive system in the planarian S.mediterranea is regulated by a gene encoding a DM domain transcription factor, Smed-dmd-1.To understand such transitions, it is important to characterize diverse modes of sex determination utilized by metazoans.Currently, little is known about how simultaneous hermaphrodites specify and maintain male and female organs in a single individual.
Consistent with this idea, the dmd-1-positive cells in the testes of mature sexual planarians did not express germ cell markers, including nanos, germinal histone H4 (ref.
In addition, dmd-1 has a male-specific role in the maintenance and regeneration of the testes and male accessory reproductive organs.
These results demonstrate that dmd-1 is an essential component of pathways responsible for inductive specification of the germline and development of a sex-specific somatic gonadal niche in a simultaneous hermaphrodite.
Here we show that a sex-specific gene, Smed-dmd-1 encoding a predicted doublesex/male-abnormal-3 (DM) domain transcription factor, is required for specification of male germ cells in a simultaneous hermaphrodite, the planarian Schmidtea mediterranea.
dmd-1 has a male-specific role in the maintenance and regeneration of the testes and male accessory reproductive organs.